Hi, I have to follow a technique called MSAP (methylation sensitive amplification polymorphism), which is a modified AFLP protocol to study genome-wide methylation. I'm don't know which adapters to be used..in the sense that the fwd and rev sequences have to be ordered like normal oligos or any end modifications are required? I have attached a publication protocol which I've been following.
Hi Neha
I can help you but I need to know what type of technology will use to visualize the MSAP perfil (eg. radioactivity, silver stain or fluorescence).
You can review the attached paper about AFLP because MSAP is a modification of this technique.
Best
Rodrigo
Hi Rodrigo
I will be using silver staining to visualize bands...i just want to confirm if any end modifications are required to synthesize the fwd and rev oligos that will make the adapter...your help is highly appreciated...
Hi Neha,
have you resolved this query? I'm also planning to do MSAP but I don't know how to order the adapters yet. Have you ordered it in Sangon? Which product type is that? I will appreciate any tips. Thank you!
I want to use these adapters:
EcoRI: 5-CTCGTAGACTGCGTACC-3, 3-CATCTGACGCATGGTTAA-5
MspI–HpaII: 5-GACGATGAGTCTAGAA-3, 3- CTACTCAGATCTTGC-5
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