My polymer is glassy solid yet stuck to whatmann fiter paper. how can I obtain my polymer?

I precipitated the polymer in methanol and then tried to filter the polymer. However, my polymer stuck to whatman filter paper. how can I obtain the polymer now?

08 September 2018 373 2 View

I was not able to isolate the product from the DMSO . Please help me how to do it?

I want to synthesize the exo norbornene alcohol as was described in the reference.Macromolecules 2005, 38, 9455-9465. the problem is that I was not able to remove the DMSO solvent. Please help me...

06 July 2018 3,925 3 View

Can I dry macromonomers with Norbornene end cap at 120 degrees centigrade for 4 hours?

I synthesized polymers of PEO with different molecular weights with Norbornene end cap. I want to dry the sample in order to perform for the Ring Opening Metathesis Polymerization. Can I dry my...

05 June 2018 2,273 4 View

Is silica gel soluble/partially soluble in acetone?

we need to purify the pyrrolidone derivatives. When we checked the TLC, the compound Has Rf of 0.6 in acetone. But other solvents showed no movement of the spot from the base line. However, when I...

05 June 2018 2,345 3 View

Can we really trust the procedures published in the scientific journals?

I found that many procedures reported in the journals are not reproducable even when same conditions are maintained? why is it so?

10 November 2017 1,852 2 View

How to prepare 1, 1-Dipheynylmethyl Potassium ?

I want to prepare DPMK initiator for my polymerizations. Please give me a pdf file of the journal necessary.

08 September 2017 384 4 View

Is the refractive index of both monomer and polymer same?

07 August 2017 4,315 4 View

How to remove moisture from the polyethers?

I synthesized polyethylene glycol. I need to remove moisture from the polymer. T tried freeze drying but it didn't work please suggest me some other method which removes moisture. Thanking You in...

07 August 2017 2,411 9 View

Please let me know about the good practical books for learning glass blowing techniques.?

glass blowing for Schlenk techniques

03 April 2016 943 2 View

What is the weight of the polymer 'polyisoprene' with a molecular weight of 10000?

please help me in calculating the weight of the polyisoprene whose molecular weight is 10000.

02 March 2016 974 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View