Can anybody give the procedure for a nitrate reductase enzyme assay?

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When to use inverse rank based normalization for phenotypes in genetic studies?

One of the assumptions in linear regression is that the data should be normally distributed. My data is skewed. I tried log, square root, and several other transformations which I could find still...

30 May 2021 2,394 7 View

Is there IPR research in Social Sciences?? Especially, in developing nation context??

I'm working on Geographical Indications in India. I couldn't get enough research publications in that domain. If any sound methods are available, kindly let me know.

11 May 2021 2,076 1 View

How to identify covariates statistically for a genetic association studies?

The genetic association studies need to be adjusted for the covariates such as Age, Gender etc. Is there a statistical way to figure out that? And is it required that all these covariates should...

12 January 2021 488 3 View

How to get TOPMED reference panel ( or south asian specific) for the imputation in Michigan Imputation Server?

Hi All, I am staring into imputation of variants for the genome wide scanning array in past weeks. Though I am able to do imputation using 1000GP3 panel using Impute2 for my dataset, I...

21 August 2020 2,846 1 View

Can anyone is having bonducellin compund?

Dear All I am doing research on Poly cystic over syndrome (PCOS), for my work I need to isolate bonducellin, for my work i need bonducellin to run hplc. kindly help me in this regard.

07 January 2020 3,450 0 View

Can we measure 25 hydroxy vitamin D in whole blood sample?

I froze the whole blood sample without separating them to serum and RBC by mistake. Is there any way to measure 25 hydroxy vitamin D in whole blood sample? If yes, is there any difference between...

31 August 2016 5,911 1 View

Which one is the best technique to find the concentration of metallic nanoparticles in solution form?

I have metallic nanoparticles in solution form. Can any one suggest best method to find the concentration?

15 October 2015 7,961 16 View

Is there increased non-locality when a system undergoes non-Markovian dynamics?

Non-locality is a curious feature, yet essentially a quantum attribute that is linked to the violation of Bell inequality of any form. It arises from the impossibility of simultaneous joint...

11 October 2015 1,286 8 View

What are the culture conditions for primary melanoma cell line, WM-115?

I'm trying to grow WM-115 but they've all lost their viability after three passages. It'd be great if you can advice me on few things: 1) What incubation condition is favoured for their growth? I...

22 April 2015 9,984 1 View

What are the immunological actions of Tuberculin PPD?

Does anyone have experience in using them as Ag in DC-T-cell culture?

23 October 2014 4,726 5 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Pragmatic inquiry research design?

Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...

28 July 2024 540 2 View