SOD specific activity (mU/mg protein) = Inhibition
percentage (%)/ V × [protein] × t.
(fridovich 1977). i
One unit of SOD activity is the amount of the enzyme that inhibits the rate of auto oxidation of pyrogallol by 50% and was expressed as Units/mg protein/min. The enzyme unit can be calculated by using the following equations:
Rate(R) = (final OD-initial OD)/3 min,
% of inhibition = {(blank OD-R) /blank OD} x 100 ,
Enzyme unit (U) = (% of inhibition/50) x common dilution factor.
[50% inhibition = 1 U]
S. Murklund and G. Murklund, Eur. J. Biochem. 47, 469 (1974).
Specific activity = ( U/mg) protein.
In case of SOD, amount of SOD required for 50 % inhibition of the test compound like NBT, pyrogallol or epinephrine is considered as one unit of SOD activity. So based on the amount of SOD for 1 unit you can calculate how much unit of SOD present in one mg of protein.
One unit of SOD activity is the amount of the enzyme that inhibits the rate of auto oxidation of pyrogallol by 50% and was expressed as Units/mg protein/min. The enzyme unit can be calculated by using the following equations:
Rate(R) = (final OD-initial OD)/3 min,
% of inhibition = {(blank OD-R) /blank OD} x 100 ,
Enzyme unit (U) = (% of inhibition/50) x common dilution factor.
[50% inhibition = 1 U]
S. Murklund and G. Murklund, Eur. J. Biochem. 47, 469 (1974).
@sonam , is it specific for pyragallol oxidation or NBT reduction formula is different ?
thx for ur replies but i need formula of sod for NBT reduction !
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