Wel, it depends on the enzyme source as you can obtain it from commercial componaies such as Novozymes or Genencor, however you can also have it from a wild type and refinine it with some effort.
As in the strategy you can run a SHF or SSF, but first you need to know your feedstock characteristict and performance within a specific petreatment so you can choose which strategy you may follow...
Thanks Jose, when the enzymes are gotten how do one calculate the FPA? Is there any equipment to do that or procedures?
The filter paper units (FPU) it is a standard protocol for enzyme activity, I attached to LAP procedures from NREL, where you can read more about the protocol for FPU and how to carry on a enzymatic saccharification with biomass.
Regarding what equipment you need for FPU, you will need a water bath at 30°C and a spectrophotometer among some reganets as glucose..
The cheapest source of enzymes (more often than not) is to grow them, and in general that means establishing a system for inoculating your substrate with one or more fungi. (It may also be possible to get enzymes from, say, adding termites(!) or rumen fluids.) The disadvantages-- and regardless of one's approach, buying commercial or "home-grown", there are disadvantages and advantages-- is that one cannot establish clear metrics where amounts (of inoculant or enzyme) are essentially indeterminate. It's the real world, after all, not the lab.
From my point of view, however, given that practical use of the biogas process itself will, without question, likewise be largely indeterminate in exactly the same way and for exactly the same reasons, it is not clear that knowing the precise amount and kind of enzymes being added would provide further real and practical effects. A/B comparisons could be made, and would be helpful, but within the complex process of biogas digestion, it is not clear that there is anything like a handful of magic, deterministic numbers.
In any case, there are a number of papers regarding the use of fungi to make lignocellulosic materials more amenable to digestion, such as "Application of Mushroom Fungi in Solid Waste Management", Jebapriya, et al, http://iirpublications.com/papers/october/ijcoa/paper%2026.pdf. I have over 60 such papers (i.e. with specific regard to using fungi to pretreat substrates) in my collection. See the attached other examples.
David House, author, The Complete Biogas Handbook, www.completebiogas.com
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