I have many plants rich with mucilages , I need to know the procedure to isolate them alone
I think boiling with hot water and then allowing to cool will give the mucilage portion if they are really in a high quantity. I have not worked on this but some of my colleagues did and they noticed the mucilaginous portion upon cooling. Hopefully give it a try and see the result. You may succeed. Gud Luck
Regards
Have you tried alcaline hydrolysis to hydrolyze proteins and nucleic acids and to saponify lipids ?
Dear Nidal
Check the following publications on separation and characterization of plant mucilage.
1. Baveja, SK, Ranga Rao KV and Arora J. Examination of gums and
mucilage as sustaining materials in dosage forms. Indian J
Pharm Sci 1988; 50: 89-92.
2. Wahi SP and Sharma VD. “Studies on suspending property of
mucilages of Hygrophila spinosa T.Anders and Hibiscus
esculentus Linn. Indian Drugs 1985; 22(9): 500-502.
There are many more references in Google. Search for extraction of plant mucilages.
Good luck.
Mucilage is generally secreted from glands which are present in fruits and leaves. This is an thick sticky material contains water, proteins, carbohydrates, minerals and lipids. You can separate crude mucilage by roiting the fruits after cutting them from middle in a horizontal vertis, pour it in a sterilized vessel. Or extract it in cold water by crushing and messing of fruit pulp or thick grossy leaves.
Check this thread on a similar subject:
https://www.researchgate.net/post/What_is_the_best_procedure_for_extracting_the_mucilage_from_a_plant_organ
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