The wastewater released from leather industries may contain a variety of pathogenic and non-pathogenic bacteria.I am present time working on pathogenic microbes.
I think we need to know first a unique DNA or RNA signature that corresponds to each individual target pathogen. Then, we can design primers for amplification of that known DNA or RNA signatures. I do not know. I am not an expert in detection of microbial pathogens. Just I am wondering?
Is best to know you target pathogenic gene. Wastewater has very broad number of bacteria. For example is better to choose pathogenic gene of salmonella than salmonella spp gene, or enterotoxigen gene of E. coli than E.coli gene
I agree with the answers given by both Kunal Dutta and Antonio Fernandes.
Dear Kunal Datta thanks for suggestion and
I am present time use for research work , 27F and 1492R primers but these are universaI primers and I need specific primers for target to pathogenic bacteria.
Dear fernandes
I satisfied your answer and good suggestion target of pathogenic gene for bacteria.
Hi! Check the following links you get some useful ideas. Follow them. Any doubts you can ask to clear.
Good Luck!!
Bacterial Pathogens and Community Composition in Advanced Sewage Treatment Systems Revealed by Metagenomics Analysis Based on High-Throughput Sequencing.
(http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0125549&type=printable)
Identification of Bacterial Populations in Dairy Wastewaters by Use of 16S rRNA Gene Sequences and Other Genetic Markers
(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC444815/pdf/2246-03.pdf)
Characterization of bacterial diversity in two aerated lagoons of a wastewater treatment plant using PCR–DGGE analysis
(https://ac.els-cdn.com/S0944501307000857/1-s2.0-S0944501307000857-main.pdf?_tid=36247eea-a341-11e7-a283-00000aacb35d&acdnat=1506488793_a73fa519145b6daaa3d8b10891e6e4ac)
A Universal Method for the Identification of Bacteria Based on General PCR Primers
(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209952/pdf/12088_2011_Article_122.pdf)
Rapid identification of bacterial pathogens using a PCR- and microarray-based assay
(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2741468/pdf/1471-2180-9-161.pdf)
Bacterial Diversity of Active Sludge in Wastewater Treatment Plant
(https://ac.els-cdn.com/S1872579109600154/1-s2.0-S1872579109600154-main.pdf?_tid=b6ee0190-a341-11e7-9081-00000aacb35d&acdnat=1506489009_84c128552a3eb66ec72381bdf0cf87e7)
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