For capsule staining I used over night bacteria culture and smear with 1% congo red . After that, dry the slide in room temperature and observe under light microscopy 100x magnification oil immersion . But I did not find good capsule layer around the cell surface. Can any one tell me better protocol for capsule staining with congo red ?
Do you fixate with 80% ethanol saturated with NaCl before congored use?
Enrico Marani actually I never used 80% ethanol saturated NACl for fixate. I used India INK and CV but result is not good. I used congo red but still i did not find capsule layer around the cell. However, can you explain saturated NACl with 80% ethanol protocol ? example 100 ml ethanol with 1 gram NACl ?
Matthew Ibbs can you tell me manvels solution protocol because I could not find their protocol. They just indicate 10% FeCl3 and 5% phenol, acetic acid like that.
For all classical stainings and solutions look into:
Romeis B (1948 there are more recent editions) Mikroskopische Technik Muenchen, Leibniz Verlag. and
G.L. Humason Animal Tissue techniques 3rd ed (1972) Freeman and Company San Francisko.
1% aqueous sodium hydroxide 0.5 ml add to 80% ethanol saturated with Na Cl 50.ml mix and use within 15 min, in other words make it fresh. Treat for 10-20 min.
Enrico Marani
I follow your protocol . Firstly, I made 50 ml of 1% aqueous NaOH with 80% etoH with Nacl . Then over night bacteria culture put into glass slide and dry with air. Then I put the solution into glass and smeared and dry. After that I used 1% congo red and dry. Observe into light microscopy. But I did not find CPS layer in my bacteria sample. Can you check.
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