Universal fluorescent primer (M13). See attached two papers for further info.
Attached #1, see the Introduction part (yellow highlight). Also refer to its citation list, paper 3.
Attached #2, see the diagram (Fig. 1) of how to use it in a PCR amplification.
We use the Universal fluorescent primer strategy with M13 sequences as described by Oetting (1995) successfully for years on LI-COR 4300. Attached one of our papers for info.
Greeting! @ Dr. Budahn.
I read your attached paper. I know your co-author Dr. Dariusz Grzebelus from Polland. We used to work at UW-Madison under Professor Dr. Phil Simon, who is a carrot breeder from USDA. We had a couple carrot papers together. Good to see that. The world is indeed small!
You work also in carrot or in an other crop? I have three different cooperations with China, but all with Brassica crops.
Best wishes Holger Budahn.
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