Hi Praveen, you can try dialysis http://groups.molbiosci.northwestern.edu/morimoto/research/Protocols/II.%20Eukaryotes/A.%20Cell%20Culture/2c.%20Dialysis%20of%20FBS.pdf

Thank you Antonio for the paper...Do you have any idea how to remove amino acids from DMEM media?

hi praveen, you could apply the dialysis system also to the culture medium i think, or you could buy a custom synthesized  DMEM without any amino acids.

Are you gonna carry out a SILAC experiment?

Hello Antonio.. Actually i want to see the role of individual amino acid in akt signaling..to do that i need to make the cells deprive of amino acids..for that i need to make the medium free of amino acids