Can any one please help me to prepare the ampicillin?

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Alejandro Martin

If it's the sodium salt, dissolve it in water to the desired concentration, filter sterilize and store at -20*C. If the free acid, add NaOH until it dissolves, then filter and store at -20*C.

Bert Lampson

Just add the anhydrous powder directly to cooled LB agar and stir until dissolved. Same for LB broth.