Can any one help in explaining the Rate Kill Assay?

06 June 2016 0 802 Report

i want to perform the rate kill assay for some of the actinobacterial secondary metabolites, i want to know the detailed procedure for performing the rate kill assay.

Badges
Science topic

Similar topics
Microbiology
Assays

More Monsumee Alam's questions See All

How to determine IC50 value of a compound ?

After plotting inhibition % and concentration (ug/ml)respectively in excel how to get the IC50 value.

31 December 2016 3,825 8 View

What are the tools that are required for16S rRNA secondary structure and restriction sites analysis?

I want to know about some tools that are required for 16S rRNA secondary structure and restriction sites analysis, as well some research papers that can help me in this regard.

10 November 2016 6,072 0 View

I want to characterize actinobacteria on the basis of its chemical properties, please suggest some methods?

i want to find the cell wall chemotype, whole sugar pattern, peptidoglycan type, fatty acid pattern, major menaquinones, phospholipid type and G+C content of different actinobacterial isolates for...

01 February 2016 9,656 1 View

Can anyone help me regarding DNA-DNA hybridization?

I want to carry out DNA-DNA hybridization for one of my strains to find its novelty. Can anyone suggest me a feasible way to carry out the technique or any laboratory where I can outsource it.

01 January 1970 4,512 0 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View

How to use Desmond in HPC ?

Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...

28 July 2024 6,553 1 View

AGILENT HPLC 1100 SERIES , any expert available please?

AGILENT HPLC 1100 SERIES operation assistance needed.

25 July 2024 10,045 2 View