How to perform Molecular dynamics (MD) simulation for corrosion system ?

Dear researchers, Greetings! Please help me to study surface of Fe(110) with Organic Corrosion inhibitor molecules using Materials Studio software. Kindly give suggestion to create a simulation...

02 August 2020 4,267 2 View

Why do I get unusual artifacts(nuclear staining) in Immunoflorescence?

I have stained WT HCT116 cells with Gapdh (green fluorescence). Fixed the cells with 4% paraformaldehyde. Washed with 1X PBST. Blocked with 1% goat serum/ BSA. Incubated with primary Ab overnight....

27 July 2020 231 11 View

No title

We have neutral protease, which gets denatured in a week even after freezing.

24 June 2018 7,564 11 View

Can u please help me to research about the recent issues in VM allocation in cloud...?

I am doing research in resource allocation in cloud computing. i need few help regarding VM allocation / resource allocation in cloud computing and some related links to carry out my research...

15 October 2017 8,553 5 View

What is the reason for lower angle shift in XRD analysis in Nb2O5 nanoparticles

The simple procedure of my Nb2o5 nanoparticles synthesis is as follows. The precursor NbCl5 and ethanol is mixed and to this solution P123 is added. then kept in a hot plate (50 deg) for a week....

04 May 2017 299 1 View

Why does the TiO2 thin film derived from sol of TTIP (Titanium tetraisopropoxide) is getting peel off after driying?

TTIP was used to form the Ti-sols at room temperature. Deposited thin films on glass slides were producing cracks and getting peel off after air drying. What may be the reason for immediate...

07 March 2017 1,441 3 View

How to calculate the vapor pressure of S?

For sulfurization process , controlling the vapor pressure of S is important.How does it vary with temperature and amount of S, how to calculate the vapor pressure of S using ideal gas law..

24 July 2016 1,499 8 View

Should you call your sample items, participants or respondents in mixed methodological approach?

16 June 2016 2,206 7 View

Concentrations of proteins to be used for assays?

19 April 2016 3,429 6 View

Can anyone suggest to me why does the absorbance range of same material differs for powder and thin film?

I'm working on CuInS2 ternary compound. Material synthesized using solid state melt growth method is confirmed by XRD and it has only CIS phase but still DRS results taken for CIS powder having...

23 February 2015 8,633 4 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View