I am looking for a proper method of dissolution for my micronized particles if any body can suggest a protocol.
Transwell® and Franz cell systems mimic the lung conditions more closely, as such systems allow particles to dissolve in a volume limited environment.
Method (for which the demo was given in my past lab):
Transfer the filter paper immediately face-up to the donor compartment of setup A or B. Setup A is the original Transwell® donor compartment. For setup B, the polycarbonate membrane is removed from the donor compartment and mild heat is used to form notches in the base in order to support the glass microfiber filter/filter paper that would be placed on it.
For both set-up A and B, the filter paper carrying the drug is placed face up into the donor compartment of the Transwell® system. This donor compartment is placed in the 6 well plate and 1.5 mL to 2 mL of dissolution medium (generally 0.5% SDS in PBS). The experiment is initiated by placing 0.1-0.2 mL of the dissolution medium onto the filter paper.
In experiments, evaluating the transfer of already dissolved drug, 10 µg of the drug is dissolved in the initiating medium (generally 0.5% SDS in PBS).
Samples of 0.5 ml are taken at different interval for set up A and set up B. You can take equivalent time points also for set up A and B.
Removed volumes is replenished with fresh dissolution medium. After the final sample is taken, the filter membranes are removed and it is washed with methanol to analyze the amount of undissolved drug.
Three to nine replicates can be performed for a given experiments. Smaller number of replicates (n=3) can be selected for preliminary experiments.
Thank you Himanshu,
Yes we are now looking for this method. We have the paper as well. But still we need to develop this method.
- Badges
- Science topic
- Similar topics
- Elementary Particle Physics
- Particle