Can any body help where can I find preparative HPLC in India.?

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Can acetic acid catalyze imidation reaction under reflux conditions?

The imidation of nitronaphthalic anhydride with an amino compound is conducted using glacial acetic acid. Will this procedure yield the desired product?

31 December 2023 9,429 1 View

How can we calculate limit of detection from CV and Amperometry?

How can we calculate limit of H2S detection from CV and Amperometry?

16 July 2023 5,326 2 View

Who invented the first DC-DC Converter? and When it is invented? Which type of converter is first invented (Buck, Boost, or Buck-Boost)?

Who invented the first Power Electronics DC-DC Converter? and When it is invented? Which type of converter is first invented (Buck, Boost, or Buck-Boost)? Please share the exact reference.

11 September 2022 1,032 2 View

How to clone a gene?

Hi everyone. I want to clone a gene in pCAMBIA1302 fused with GFP. The restriction sites that I can use are NcoI, BglII, SpeI. Bt the problem is all three restriction sites are present in the...

14 July 2021 7,091 3 View

E.coli expressed truncated Bacillus thuringiensis Cry protein activation and solubilization?

I have expressed truncated bt protein in e.coli. codon plus strain, it expresses very well but my protein dumped into the pellet. For solubilization and the activation of pellet protein, I have...

07 July 2021 2,083 0 View

How do I calculate carrying capacity of herbivores of a certain forest area???

I have above ground biomass values of fodder species of the certain area.

07 April 2021 8,832 3 View

How to compare two Alpha Co-eff (same measure) in two indep samples in SPSS?

My samples are different in size and I need to see if the alpha coefficient in one group is significantly different from that in the other group. My data are in SPSS (I am not an R user,...

28 February 2021 3,059 4 View

How to extract protein after trizol RNA and back extraction of genomic DNA?

I have limited amount of samples, so I am trying to get RNA, DNA and protein from the same samples. I used Trizol reagent for RNA isolation, then use back extraction buffer for genomic DNA...

13 January 2021 1,834 2 View

How to obtain a mineral map using Hyperion image in ENVI or in Erdas Imagine?

Input is a Hyperion hyperspectral image. I followed steps Preprocessing, MNF, ppi, nD visualizer and SAM. Also spectral anayst used. I found minerals by spectral resampling of imagery spectral...

23 November 2020 9,175 4 View

How to generate Ground Truth image to use with Hyperion hyperspectral image if it is not available?

I am working on Hyperspectral image of hyperion sensor for mineral mapping? How I can generate mineral map for it?

29 December 2019 5,413 3 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View