Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Dear all, Iam currently working on Dc Microgrid model for electrical vehicle charging. Can any body give good IEEE research paper on this topic?

please give any suggestion?

04 February 2021 4,837 5 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

What does the change in D-peak position in Raman spectroscopy of carbon materials indicates?

We have carried out high-pressure Raman characterization on some of our samples. After fitting, I have observed a change in D-peak position with increasing pressure. What does this change...

20 January 2021 853 3 View

How to determine the genetic potential of the genotype in terms of fruit size and weight?

In addition to genetic and environmental factors, fruit size is also influenced by crop load, rootstock, orchard planting system/tree training system, pollination, water and nutrient management,...

13 January 2021 9,309 4 View

Is it possible to ligate two restriction ends those have no complementary bases?

In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...

01 January 2021 3,271 4 View

Is it machine learning models that have replicability capability?

We are doing the prediction of soil nutrients using spectral information by applying machine learning models. More than 300 soil samples were used from one district for analysis. We got the...

21 December 2020 9,663 3 View

How can I analyse and compare crop yield under different irrigation methods?

I have to compare yield parameters of a tree crop grown under three irrigation methods. But these systems are already established separately in three adjacent fields. Since I can not follow a RCBD...

21 December 2020 3,065 5 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Why are the modelled and circuit system different in response for controlled buck converter?

[Images are attached at bottom] I am currently working on a buck converter controlled by two different control methods, Reinforcement learning applied to find PID values & a lead-lag...

16 February 2021 5,060 10 View

How to eradicate the deteriorated contact resistance in the electrical or electronic devices, like SCR or contactor' pin cable ?

Through years operation, some of the devices' pins connection gradually become worse and finally burn out due to the high temperature at the connection point. We can check out these risk points...

04 February 2021 7,401 5 View

What if we go for one single global currency?

What if we go for one single global currency? than every country has its own currency. Currency exchange commission etc. (Advantages/Disadvantages)

04 February 2021 8,937 3 View

Is there a generalized method to derive current-mode model of any DC-DC converters?

Hi all, As new DC-DC converters are being proposed, associated control method should be proposed and based on the small-model signal analysis of the DC-DC converters. Using average space state...

06 January 2021 7,247 3 View

Electronic nose in perfume industries?

give me the details of the use of E-Nose in perfume industries and its applications

30 December 2020 3,849 1 View

What is your expert opinion/experience/suggestion about the new variant of Novel Corona Virus (COVID-19)?

Dear Research Gate Members, Nowadays, everyone is talking about the new variant of Corona virus COVID-19 and its affects are alarming throughout the world. Please, share your expert suggestions...

29 December 2020 7,555 20 View

Have you observed the improvements in Online Meeting Platforms during COVID-19? In this context, how will you compare Google ZOOM & Microsoft TEAMS?

Dear Research Gate Members, Online Education / Distance Learning and Work From Home (WFH) are the ultimate new realities for many in the world due to Pandemic Novel Corona Virus (COVID-19)...

29 December 2020 9,866 37 View

Why mutual inductor takes high current at no load condition?

I simulate an inductive wireless power transfer system in matlab. I set the primary and secondary mutual inductor resistance is 0.3ohm. So when i run the circuit it took high current at no load...

29 December 2020 9,536 3 View

What is the appropriate k points number for band structure calculation ?

Hi everybody, I am a new user wien2k 14. I am trying to optimize the electronic structure of ternary alloy (with 8 atom in the unit cell) but I am not sure about the good k points number. Thank you.

14 December 2020 7,719 8 View