Hello,
I am currently constructing E-coli based in vitro translation system that usually utilize ammonium glutamate in reaction salt solution according to recent publications and published methods. However, I am unable to purchase ammonium glutamate. so, I read an old publication using ammonium acetate in the translation mixture, here is a link Article E. coli -Based In Vitro Transcription/Translation: In Vivo-S...
would this change gave the same required results?