I did immunohistochemical staining with Calretinin (D29k) and got a lot of relatively large cells stained, mostly in the hillus of the hippocampus. My aim at the begining was to label GABAergic interneurons but after a lot of literature review, I found different authors saying different things about the identity and characteristics of Calretinin-positive cells in the hippocampus.
I doubt whether they are newborn neurons because only very few (and smaller ones) were found lying along the subgranular zone where I expect newborn neurons to be found. However, another paper said those calretinin cells of the hillus do not produce GABA. Again, some other reports said these cells are mssy cells.
Can someone, enlighten me on the identity of these hillar, calretinin cells? Any help will be appreciated.
Calretinin staining is used to label mossy neurons in the hilcar region in mice. New-born neurons are also calretinin-positive, but the staining is weaker and they are located adjacent to the grandule cell layer.
Hi Samuel,
What happen with your search for calretinin positive cells in the hilus. Did you try to co-label with a GABA marker like GAD65/67 to check colocalization?
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