Samuel O Adeosun

6 Questions 10 Answers 0 Followers

Questions related from Samuel O Adeosun

Why is HEK293/293T used for neuroscience-related studies?

I know that HEK293 and HEK293T cells are supposed to be human embryonic kidney cells but I have always wondered why these cells are used in studying almost every kind of neuroscience related...

10 October 2015 3,056 5 View

What proteomic technique is available for high throughput assessment of post-translational modifications of proteins?

I'm trying to understand the post-translational modifications of a certain protein at different conditions. The protein can be immunoprecipitated from mouse tissues. Is there a proteomic (or...

04 April 2014 3,535 9 View

Has anyone used the iBind Hands-free Immuno-detection system?

I came across the description of the iBind system from Life technology and I find it interesting. I'm wondering whether someone has used the device so as to know whether it works really well as...

03 March 2014 2,314 4 View

Induction of Caspase 3 by Staurosporine in mouse primary neurons: Dosing & Timing?

I'm trying to induce caspase activation in primary neurons with staurosporin treatment. Can someone help with what concentration I should start with and duration of treatment to see an easily...

01 January 2014 1,671 12 View

Calretinin-positive cells in the Dentate gyrus; Mossy Cells, Interneurons or Newborn neurons?

I did immunohistochemical staining with Calretinin (D29k) and got a lot of relatively large cells stained, mostly in the hillus of the hippocampus. My aim at the begining was to label GABAergic...

04 April 2013 4,723 2 View

Western Blot Protocol for Amyloid beta

Can anyone help with a working protocol for amyloid beta detection in mouse brain by western blot? I've tried using two different antibodies (abcam and CellSignalling) but none of them worked. I...

01 January 2013 7,262 6 View