Suppose I have a fluorescence intensity image which having data like 2000*2000*8unit. How can I calculate the Ex, Ey and Ez of that intensity image? Any suggestions???
@ Peter, I think Micheal is right we cannot do that. I have read manual we can fluorescence intensity data in the form of x*y*8unit. and from the intensity, i dis not find any formula which converts total intensity into Ex, Ey.
@Michael Päch, I am not sure maybe there is. because S0 is the first stokes parameter which describes the total polarization intensity which is S0=Ex^2+Ey^2 and 2nd stokes parameter S1=Ex^2-Ey^2, from these two formulas we can find out the Ex and Ey. Now I am confusing about S0 which is polarization total intensity and total fluorescence intensity I.
@Peter Kapusta, please don't deal everyone with the same scale, a good researcher and writer is who can explain the things so clearly that everyone can understand. Actually, I have performed these things with the finite difference time domain method but that was for polarization parametric indirect microscopy which is of super-resolution microscopy. we measured some direct field intensity and then some indirect measurement from that direct results (in that indirect measurement we calculated some parameters). which have a higher resolution I want to the same with fluorescence.
@ Peter Kapusta sorry for my orthodox thinking. Thanks for your reply. I don't know, but one thing I know that we can calculate if we know the average of polarization intensity Idp, then we can calculate some polarization parameters like stokes S0, S1,S2 and S3. and from that we can find out the Ex and Ey. Please have a look at the image attached and article.
https://www.osapublishing.org/oe/abstract.cfm?uri=oe-22-17-20446
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