Fourier-transform infrared spectroscopy, also known as FTIR spectroscopy, has made its mark as a very versatile technique of analysis with utility in a range of scientific research areas in different disciplines.

No, it is not possible to calculate the % of alpha, beta, and random content without using software. Protein secondary structure predictions rely on algorithms that utilize a variety of factors such as sequence information, chemical properties, and other related factors to make an accurate prediction. Without the help of software and algorithms, it is not possible to accurately calculate the % of alpha, beta, and random content.

sir, I have just data of FTIR spectra of my respective protein and their nanomaterial. I couldn't find any help related to calculating secondary structure.