Convection-enhanced delivery (CED) system is a powerful treatment for directly bypass BBB in brain tumor therapy. I try to set up the system and the in vivo animal model. In the begin, we use TMZ ( Temozolomide) to test the IC50 value of U87 cells. the IC50 of U87 cells is 250 uM. however, I have no idea...how to decide/calculate the concentration of TMZ ( I think the concentration should be higher than 1000uM...) for CED injection (10ul, 0.5ul/min,directly inject drugs into brain tumor, not IV or IP injection, nude mice, xenograft model)?
is anyone have similar experience or suggestions? I really hope I can have a right direction to do the animals experiments without scarifying unnecessary "testing animals...."
Hi
Please go over this article http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=19875&pubmed-linkout=1
Route of administration (injection) is also considerable factor
DEAR Srinivas: Thanks for your kindly reply! but This paper discussed the dosage of IV injection. for CED experiment, drugs will be directly injected into the brain tumor site without the metabolism by liver or spleen.... so....I think that dosage will lower than IV injection. thank you very much~~ I still appreciate for your great helping!!!
Hi
Indeed, you need lower dose than they previously used. You may use 80% less
Good luck
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