Buying/designing a miRNA primer for sequence length 15 bases?

13 January 2017 1 5K Report

Hi,

I am validating some miRNA results by RT-qPCR, and need to design primers for my sequences. Companies like Qiagen and Thermo require a minimum of 16 or 17 bases for a custom miRNA primer assay. All six of the sequences I need to test are only 15 bases. Have any of you encountered this, and how did you solve the problem?

For example, a couple of my miRNAs/sequences are:

mir-1382 GGAGGGCCGCTGCGG

mir-2861 CGGCGGGCGTCGCGC

Nell Bekiares

Thanks. My plan is to do qPCR, however, so I do need primers that will work with an RT-qPCR kit. Any additional suggestions?

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