I am cryomilling my cells and need to establish the best buffer to solubilize my protein of interest. Does anyone have any ideas of buffers to use or researches to use to determine the best buffers to use?
Dear Josiah Flaming
Pls. find the attached files and the references, good luck
https://academic.oup.com/nar/article/42/2/e13/1030103/An-optimized-streptavidin-binding-RNA-aptamer-for
Hoping this will be helpful
Regards
Prof. Houda Kawas
http://www.nature.com/ismej/journal/v7/n1/full/ismej201272a.html
https://books.google.com/books?id=Fv73wuh1dNUC&pg=PA230&lpg=PA230&dq=protein+extraction+of+cryomilled+cells&source=bl&ots=wy03YX3VJG&sig=WpzWlII33ihakXpHUeH0xNzDQAI&hl=ar&sa=X&ved=0ahUKEwjhjbuAo7fSAhWC7BQKHVLuBO44ChDoAQggMAE#v=onepage&q=protein%20extraction%20of%20cryomilled%20cells&f=false
https://www.jove.com/video/54518/-cryomilled?language=Arabic
http://www.biotechniques.com/BiotechniquesJournal/2015/March/Affinity-proteomics-to-study-endogenous-protein-complexes-Pointers-pitfalls----preferences-and-perspectives/biotechniques-357167.html?pageNum=2
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226390/
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-cell-lysis-and-protein-extraction.html
http://f1000.com/prime/ext/727181777?referrer=GOOGLE
http://fenyolab.org/publications/pdf/15_hakhverdyan_nm.pdf
Article Affinity Proteomics to Study Endogenous Protein Complexes: P...
Also
https://books.google.com/books?id=GJwjDQAAQBAJ&pg=PA8&lpg=PA8&dq=protein+extraction+of+cryomilled+cells&source=bl&ots=3grd41i6S0&sig=D-p2V-QnmL9Ah7YgqhBGxapGr2g&hl=ar&sa=X&ved=0ahUKEwjhjbuAo7fSAhWC7BQKHVLuBO44ChDoAQhZMAg#v=onepage&q=protein%20extraction%20of%20cryomilled%20cells&f=false
http://www.sciencedirect.com/science/article/pii/S009286741301297X
https://books.google.com/books?id=mQQ4AAAAIAAJ&pg=PA243&lpg=PA243&dq=protein+extraction+of+cryomilled+cells&source=bl&ots=Ied8F2fnxk&sig=VC3vJoyJQe0ekZ7ybsybBYiUge4&hl=ar&sa=X&ved=0ahUKEwir5dWRpbfSAhWFYpoKHcIFCRk4FBDoAQgqMAI#v=onepage&q=protein%20extraction%20of%20cryomilled%20cells&f=false
http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002365
http://www.worldlibrary.net/articles/eng/Cryomilling
http://research.omicsgroup.org/index.php/Cryogenic_grinding
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