We prepared tissue sections of striatum from excised brains of rats and stained with haematoxylin and eosin (H&E) reagents for the purpose of observing histopathology alterations in the stained tissues. My question now is, can we possibly count the the total number of tyrosine hydroxylase (TH)-positive neurons of substantia nigra in the H&E stained tissues? We would appreciate answers with reasons and references (if available) and any other available guide that could give clarity to the question.
Hello,
do I understand you right: You want to do Immunos on your stained H&E-Slides?
That I cannot answer!
Do you have parallel sections? These could be stained for TH via immunohistochemistry and then counterstained. Therefore, I would recommend Nissl-stainings.
Kind regards
Stefan
Many thanks Dr. Stefan Jean-Pierre Haas For your answer. It is quite helpful.
Kind regards
Sunday.
Many thanks Dr. Adwitia Dey
for your contribution.Regards,
Sunday
H & E staining cannot provide the details you want about counting TH-positive neuron. It is better to do IHC or IHF
Many thanks Dr. Ibrahim Olabayode Saliu for your response. It provided quite an additional clarity to the subject que...
Regards,
Sunday Josiah
Neuronal count for H&E staining cannot be accurate. IHC would give better result required for counting TH-positive neuron
Thank you Dr Ogunlade Babatunde for your answer. It is much appreciated.
Regards.
Sunday
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