The diagnose of Entamoeba histolytica stages in stool samples using microscopy most often give rise confusion of some cells like pus cells and other round cells which are resemble to cysts of Entamoeba histolytica, Moreover sometimes its difficult to the lab. technician to distinguish between the pseudopoda of this Entamoeba with other non-pathogenic rhizopoda, So I think this procedure is golden one to solve this lab mystery, why?