I want to measure the activity of propionyl-CoA carboxylase (a mitochondrial enzyme found in many tissues). I will be extracting the enzyme from frozen tissue and need to store this somehow. Extraction and store the crude enzyme from tissue is done in either some kind of phosphate buffer (potassium phosphate or Tris phosphate, so around pH 7.2) or water only (> pH 8). The same goes for compound standards - some papers store stocks in buffer and some in water. However, I read that CoA solutions are most stable frozen at pH 2-6. I am not sure what would be best, buffer or no buffer and what pH to store both standards and enzyme source? I think a buffer is always better than water, especially with enzymes, but I would like to hear other expert opinions.
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