pls mention your primer sequences used for this PCR

http://www.antibody-antibodies.com/fr/product7841-search-RABBIT%20ANTI%20GABA%20A%20RECEPTOR%20ALPHA%205%20(C_TERMINAL).html

Alpha 1 subunit:

rGABRA1.RT-F

5’ TTCACCAAGAGAGGGTATGC 3’ 20mer, Tm 56OC, GC=50%

rGABRA1.RT-R

5’ CGGCTTGGCAACTATTGCTA 3’ 20mer, Tm 56OC, GC=50%

Reverse complement:

5’ TAGCAATAGTTGCCAAGCCG 3’

Amplicon = 153 bp

Alpha 5 subunit:

rGABRA5.RT-F

5´GAAGGCCTTGGAAGCAGC 3´ 18 mer, 57oC Tm, 61% G+C

rGABRA5.RT-R

5´GTACAGCCTCAATCAGAGCAT 3´ 21 mer, 57oC Tm, 48% G+C

Reverse complement:

5´ATGCTCTGATTGAGGCTGTAC 3´

Amplicon = 167 bp

Hi Sorry for delayed reply. I have analysed your primer details, your Alpha 1 subunit is specific for both mRNA and DNA. Hence it gives amplification around 1800bp (DNA as template. see the power point file attached . I hope this will solve your problem.

I am happy to assist you further.