Hi every body
I wondring about using Boiling method DNA extraction is helpful for doing real time PCR for identification of bacteria culture (e.g.corynebacteria and pasteurella spp).
hi our collegue our team request to publish free publiication paper in botany with short time publication for a plant from ulmaceae family with best regaeds
11 December 2018 8,018 2 View
Hi every body I submit RHDV (Rabbit hemorrhagic disease virus) paper for OIEpublication . They requested International OIE publication reviewer(not from Egypt) for (Rabbit hemorrhagic disease...
10 November 2018 9,253 0 View
I did NMR H proton and C13 are enough to identify total methanolic extract plant of Natural products . I am not fully identify all the components. if any possibilties for further identification?
10 November 2018 7,633 0 View
A commercial kits using comprtitive ELISA for RHDV
09 October 2018 8,702 0 View
low and degraded Mycobacterium DNA with special reference to bovis one
08 September 2018 272 2 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....
02 March 2021 285 2 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...
28 February 2021 400 1 View