Why can I not enter the title of the journal?

When I upload articles the details page does not allow me to enter the names of lesser known journals. It says "type to search" but because the journal is not on the list, I am left with a blank...

26 January 2021 2,988 3 View

Has anybody used computational linguistics-based, AI-assisted analytics for their research like the covid-19 study below?

16 November 2020 8,316 3 View

Is it possible to convert WRF output data (netcdf) to grib?

I'd like to use WRF output data to force a land surface model (HRLDAS). HRLDAS requires grib2 forcing data and I have so far been unable to convert from netcdf to grib. There are a number of tools...

17 March 2020 8,962 6 View

Is it possible to find distance of objects in an image using AI?

I am working on a project for my school, the projects are unique and based on real world problems so there really could not be a solution. So here it goes. I have a bunch of photos of fields,...

03 March 2020 6,951 5 View

Are you a motivational intervieiwng trainer/practitioner/researhcer based in Western Australia?

If you are focussed on (or just interested in MI) and based in WA please get in touch- I am interested in potential collaborations for practice research and training, kind regardsTrevor.

05 February 2020 6,084 1 View

Germany on chain: National Blockchain Strategy Released - Thoughts?

Article Link: https://medium.com/@philippsandner/germany-national-blockchain-strategy-released-1420e9d066f7

18 September 2019 5,237 4 View

Have your heard of Deloitte's new term "The Symphonic Enterprise"?

"In the symphonic enterprise, the old lines become blurred, thus creating a diagonal view that illuminates new business opportunities and creative ways of solving the most complex problems via the...

19 August 2018 3,894 3 View

What is the best way to preserve (fish) tissues for PacBio sequencing?

04 March 2017 6,232 3 View

Moving from cyclic voltammetry to bulk electrolysis for a mediated enzyme reaction. How do I work out what mediator concentration I should use?

23 October 2014 1,228 3 View

Does anyone know of a molecular biology protocol for replacing a large domain of a protein (~120 AA) with a small loop (5 amino acids)?

My protein contains two equal sized domains of about 120 amino acids each. I have designed an engineered protein where one of the domains has been replaced with a small loop. I do not know how to...

15 October 2014 6,552 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

How does an AC-DC power supply Flyback Converter Based USB Charger Model using Matlab/Simulink looks like?

I am required to learn about Flyback converters and I got stuck not knowing to full design of the power supply flyback converter Based USB Charger Model using Simulink, especially the design model...

25 February 2021 5,435 2 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

What effect does the large temperature difference between the two primers have on PCR, including real time PCR?

What effect does the large temperature difference between the two primers have on PCR, including real time PCR? If I did not get a result from real time PCR, is it possible that my problems are...

21 February 2021 4,094 7 View