Hello,
I am doing LIVE/DEAD staining of biofilms on different surfaces and taking z stack images by CSLM ( Olympus Fluoview FV1000).
Then I am using COMSTAT to estimate biomass thickness roughness coefficient etc.
I am following the procedure detailed on COMSTAT 2.1 manual for making the stacks and image conversion and so, but I am getting different values for these parameters depending on the channel (red of green), and the two value are sometimes very different.
Is this usual or I am doing something wrong?
Many thanks
I am also facing similar kind of problem. The values I am getting for red channel or green channel are opposite of what I could see visually. What may be the possible issue?
Md. Ashrafudoulla Convert the images to 8 bit-OME Tiff. The issue will be resolved.
For more details you can follow this article: Article Microscopic techniques to evaluate the biofilm formation abi...
Each stack should be converted? Is there a batch ( channel ) conversion?
Giorgiana M. Catunescu
The paper is not accessible. Can you send the pdf copy?
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