Have anyone tried to backbone digest Lenti-multi-guide with BsmBI for cloning?

Hi. I have planned to use the plasmid Lenti-multi-guide (Addgene Plasmid #85401). for mig cloning, and BsmBI as restriction enzyme...

21 October 2020 5,131 3 View

How to concentrate cytokines in supernatant for ELISA?

I have problem to detect different cytokines in supernatant after the treatment of cells with LPS or TNF alpha by ELISA. Can you suggest me the way for concentration cytokines in cell supernatant...

01 May 2019 6,199 8 View

RNA isolation from a small sample (specific cell population)

Does anybody have experience with isolation of RNA from a small sample? I am trying to magnetically separate CD4 cells from whole blood and thereafter isolate total RNA from that small cell...

26 June 2017 3,427 3 View

Does anybody know efficient method for HIV RNA isolation from plasma sample for NGS?

Hello, I am seeking for a method for isolation of HIV RNA from plasma sample. I am doing NGS so the isolate has to be highly pure (without any human or bacterial DNA/RNA). I tried standard kits...

29 March 2017 3,236 3 View

How do I treat tissue for paraffin embedding after its been fixated in Carnoy's Solution?

07 June 2015 5,959 8 View

How can I fix the linear command that gives me an empty logistic output?

I am trying to run the linear association analysis with a separate phenotypic file where I have 12 quantitative phenotypes. When I try to run the analysis I get the message that I have 0 cases and...

13 April 2015 7,838 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View