Have anyone tried to backbone digest Lenti-multi-guide with BsmBI for cloning?

Hi. I have planned to use the plasmid Lenti-multi-guide (Addgene Plasmid #85401). for mig cloning, and BsmBI as restriction enzyme...

21 October 2020 5,131 3 View

Bio-polyamides for application in textile sector?

What are the oil based inputs for production of bio-polyamides for application in textile sector?

29 February 2020 7,286 1 View

How to concentrate cytokines in supernatant for ELISA?

I have problem to detect different cytokines in supernatant after the treatment of cells with LPS or TNF alpha by ELISA. Can you suggest me the way for concentration cytokines in cell supernatant...

01 May 2019 6,199 8 View

RNA isolation from a small sample (specific cell population)

Does anybody have experience with isolation of RNA from a small sample? I am trying to magnetically separate CD4 cells from whole blood and thereafter isolate total RNA from that small cell...

26 June 2017 3,427 3 View

How to measure the percentage monomer conversion during a polymerization process?

Dear fellow researchers, I am currently performing a polymerization process and wish to know the overall percentage conversion in regards to my initial monomer content. I have seen in some...

15 April 2017 6,700 11 View

Does anybody know efficient method for HIV RNA isolation from plasma sample for NGS?

Hello, I am seeking for a method for isolation of HIV RNA from plasma sample. I am doing NGS so the isolate has to be highly pure (without any human or bacterial DNA/RNA). I tried standard kits...

29 March 2017 3,236 3 View

How do I treat tissue for paraffin embedding after its been fixated in Carnoy's Solution?

07 June 2015 5,959 8 View

How can I fix the linear command that gives me an empty logistic output?

I am trying to run the linear association analysis with a separate phenotypic file where I have 12 quantitative phenotypes. When I try to run the analysis I get the message that I have 0 cases and...

13 April 2015 7,838 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View