I was culturing Bifidobacterium bifidum strain. I am using Bugbox suplemented with Ar gas which creat anaerobic environment and then i culture the broth and plates of bacterium from the start. At start i did't get enogough colonies but after successive cultruring there were alot and i started further experiments. Now some of my collegues asked if i have confirmed the bacterium if its the correct one or not. So i started to confirm while using PCR. But i did not the result now. I am worried if my culturing condition was not appropriete?.. After culturing i keept the plates wraped with a membrane usually used in labs and for broth airtight tubes and wrapped with the membrane and put in normal aerobic incubator. is that the right way to culture. ?