Best way to remove water from from vacuum pump?

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Publication duplicating concers?

Dear All, I found a paper related to my work. I will do the same thing except I will change the reagent they use. For example, instead of using reagent X, I will use reagent Y. They use gold...

25 June 2024 5,799 0 View

What learning analytics model is the best when it comes to improving student performance under any condition?

There is research into the models individually, but not one seeking to find an overall best one suited for any condition.

23 June 2024 5,641 3 View

How can I purify filamentous fungi from leaf tissue?

I want to perform PacBio RNA-seq of a filamentous fungal isolate during multiple infection time-points in barley leaf. I was told that PacBio RNA-seq would not be viable due to the low...

12 June 2024 4,027 1 View

Help me with plasmid concentration calculations?

Hello everyone, My question for sure is silly, but I have been arguing with my labmate about this. If our plasmid concentration is 0.776 µg/µL and he wants to make a final concentration for...

10 June 2024 9,942 5 View

How can i upload a document?

I want to upload my research paper here, how can i do it?

14 May 2024 7,579 2 View

How to prepare a perfusion buffer and calculate the concentrations of each enzyme?

I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...

02 May 2024 4,928 0 View

What key differences are observable in the total assay time between DI-60 and manual slide review for red and white blood cell morphology analysis?

I ask this question with regards to the total assay time because I am interested in what aspects do the DI-60 and manual slide review differ when it comes to reviewing patient samples and how do...

11 April 2024 1,686 0 View

How to set up a PCR protocol?

I have designed 2 primers Now I need to set up the PCR protocol. First: I need to know how much of each primer, H20 and GoTaq® G2 Hot Start Taq Polymerase to put in the mix. We usually put a...

28 February 2024 213 2 View

Genomic DNA qPCR protocol and analysis?

Hello All, I have several questions about qPCR. I will explain what I have done to give you a clear picture. I have 2 kidney samples. 1 wildtype and 1 KO for a specific gene. We have extracted...

25 February 2024 6,331 2 View

Relative quantification of genomic DNA qPCR?

I have extracted genomic DNA from 2 tissues. 1 wild type and 1 KO. I havd designed primers that should amplify a region in between the two loxp sites. I want to validate the effectiveness of the...

22 February 2024 9,660 0 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

Do you think can be any gas and oil bearing rocks in Eastern part of Iran?

I want to know more about petroleum deposits in Iran.

02 August 2024 8,725 3 View

Heavy Crude oil PVT Data?

Hello i need heavy oil PVT data having viscoisty of 2000-3000 cP. If someone have it or research papers about it, kindly share it with me Thanks

25 July 2024 6,940 1 View

Can we isolate microplastic from sludge sample without using vacuum filter..?

isolation of microplastic from sludge sample using centrifugation ..

23 July 2024 6,418 0 View

What are the most PROBLEMS of HYBRID nanofluid in oil industry ?

explain your idea

23 July 2024 1,197 0 View

Do you think can be any gas and oil reservoirs in central of Iran?

I want to know more about gas and oil reservoirs in Iran.

14 July 2024 3,350 2 View

Why does total carboydrate content decrease when increasing the vacuum degree?

I have conducted a survey on the "effect of vacuum drying conditions on carbohydrate content of angelica.S". The result show that, when increasing the vacuum degree, the carbohydrate content first...

13 July 2024 592 1 View

How am I supposed to find justifications for the unusual fatty acid profile in broiler diet?

I've obtained a fatty acid profile of broiler diet comprised of SFA 48 to 93%, MUFA (22 to 40%), PUFA (non determined to 10.4%. The PUFA composition is mainly n-6 and almost no value for n-3. The...

08 July 2024 9,165 4 View

Can vacuum cleaners spontaneously explode?

We assume that vacuum cleaners can explode spontaneously. Very few catastrophic accidents due to vacuum cleaner explosions have occurred in the world recently. We also assume that the mechanism or...

03 July 2024 4,846 2 View

Determination of fatty acids in fish?

I measured the fatty acids in the fish, using the internal standard method in the national standard method, I first extracted the fish oil with cable extraction, please ask me how many grams of...

02 July 2024 900 1 View