Best way to dry p-toluenesulfonic acid monohydrate?

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Where can I get my material characterized at a low cost in mumbai ?

I am currently working on biochar as a low cost adsorbent, I would like to know the elemental compositions as well as it's surface area and morphology hence i would like to get BET, XRD, XRF, CHNS...

20 October 2023 8,013 0 View

Experiences with thiazole orange-conjugated probes in qPCR?

Does anyone have any experience with using TO-conjugated probes in qPCR? Glen Research makes an thiazole orange NHS ester, and the cited literature claims that it acts both as a minor groove...

22 June 2021 760 0 View

Should I use split plot ANOVA?

I have 36 fish tanks - 3 age levels (post larval, 1 & 2 year),12 tanks per level. I apply 2 treatments with two levels each treatment; temperature (cold, warm) and feeding frequency (1 x day,...

17 March 2018 6,308 4 View

Near diploid human cancer cell line?

I am currently looking for a near diploid human cancer cell line, such as HCT116 and U87-MG. Preferably XY with modal chromosome # between 40-50, "normal" DNA repair pathways, and able to easily...

28 July 2015 6,407 2 View

Why is my G418 selection not working?

I am trying to create stable GFP CHO cells using G418 as a selection agent. I carried out a dose response curve and determined 0.6 mg/ml would completely kill cells by day 7. I used 0.8 mg/ml for...

11 May 2015 2,738 10 View

Does anyone know of a qPCR method which can distinguish between integrated and extrachromosomal plasmid DNA?

I am looking to quantify plasmid integration very soon after transfection into mammalian cells. Therefore I need a qPCR method that can distinguish between integrated and extrachromosomal plasmid....

24 February 2015 8,775 22 View

Has anyone encountered issues with MCF10a not incorporating BrdU?

Hello All, I am trying to do a simple fluorescence plus Giemsa stain/acridine orange differential stain for chromosomes. I have had great success in the past using MEF/CHO/and various cancer cell...

10 December 2014 6,904 2 View

Has anyone had an issue with Gibson Assembly not connecting a plasmid and a single insert?

Hello All, I am currently struggling with getting a Gibson Assembly to work. I am trying to insert a 2.5 Kb insert into a roughly 12 Kb plasmid (modified pLEX-MCS). I have had the G.A work in the...

01 December 2014 2,057 6 View

Nonspecific PCR products and faint bands with Q5 polymerase - any thoughts?

Afternoon everyone, I am currently using Q5 polymerase from NEB to add restriction sites to an insert in a pBabepuro plasmid (~5400 Kb vector and 2.2 Kb insert). I have designed the primers to...

13 October 2014 893 3 View

Does anyone have the sequence for the pBabepuro-MYC-ER plasmid?

Hello, Addgene does not have the complete sequence published. Also, does anyone know if myc is truncated on both the N and C terminus, a primer designed to target the start of the myc CDS,...

10 September 2014 5,726 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Is the black sediment consider as sand? If not, how can I filter it out?

Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...

30 July 2024 557 2 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

Where to start the Analytical model for Dry sliding wear of metal surface ?

I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.

29 July 2024 6,115 0 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

23 July 2024 3,891 0 View

Can sulfuric acid be used to prepare anthrone reagent when it has oxidized and changed color in reaction to light?

Sulfuric acid has changed color to yellow.

16 July 2024 6,964 0 View

Issues with Malaria Coated Nitrocellulose Membrane Releasing Moisture?

Hello, I am currently working with Malaria Pf/Pan nitrocellulose membranes coated for malaria detection. After drying the coated membranes in an Incubator (overnight at 37°C), I've noticed that...

16 July 2024 7,937 1 View