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When Using SYBR Green for Flourescent Detection of LAMP Products what causes an Overshoot in Flourescence Reading?

The Graph below shows an example of an amplification curve that shows an overshoot in fluorescence. I am using SYBR Green, BST polymerase, and 6 LAMP primers in the mixture and amplifying at 65...

14 July 2024 1,483 0 View

What is social license to operate and consent in project?

Before entering a project, social license and consent from the community of the said area is very crucical as it determined whether the projects is accepted and viable to the community and...

14 July 2024 5,319 0 View

How do I compute SEM for percentages using SPSS?

I'm evaluating the reproductive performance of quails. I have the fertility, and hatchability percentages for six different sire groups and would like to determine the significant difference...

14 July 2024 2,203 5 View

How do I fix the MS NET.Framework 1.1.4322 condition for installing PowerMarker software?

I have been trying to install PowerMarker on my Windows 10 PC but it is not working. One of the requirements for PowerMarker installation is MS NET Framework 1.1.4322 condition, however, Windows...

07 June 2024 1,553 1 View

Can Artificial Intelligence (AI) replace human creativity in some fields and careers? You can give examples?

In this presence age, AI is being used in many areas of human endeavour. Be that as it may, can human creativity, empathy, and complex problem-solving abilities be replaced with AI in any field?

04 June 2024 7,327 10 View

Mentorship and Collaboration in Medicinal and Organic Chemistry ?

I am looking for for mentor in medicinal or organic chemistry to help guide me in my research and career development. I am also open to collaborating with researchers who share my interest. If...

26 May 2024 5,186 2 View

In practice-led research, any experience related to painful and reflectivity?

A researcher always have plainful, instead of joyful, experience when they reflecting of their action taken. I, as an autoethnographer, came across of this journey, and the result is...

19 May 2024 7,348 2 View

Why Raman spectroscopy of 785 nm cannot yield proper Raman spectrum for carbon based materials like graphene?

I cannot get the Raman spectrum for carbon based materials like graphene in Raman spectroscopy of 785 nm . What can be done for obtaining the proper Raman peaks for carbon based materials in 785...

15 May 2024 3,410 2 View

The success and challenges of wildlife policy and legislation: a case study of Nigeria?

am working on a seminar topic

09 May 2024 9,788 0 View

What is a great thesis on "The Theology of the Holy Spirit in the Twenty First Century"?

Yes, we know the roles and responsibilities of the Holy Spirit, but I want to go a little deeper in my thesis or even research on the Holy Spirit, can anyone help me?

14 April 2024 9,636 8 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Would you like to join our meta analysis research team?

I am excited to announce an opportunity for dedicated researchers to join our dynamic team for an ongoing meta-analysis study. If you are passionate about research and have a keen interest in...

01 August 2024 6,737 1 View