Can you suggest some plant transformation vectors that I can use for the expression of GFP/YFP-tagged protein of my interest?

I want to generate stable Arabidopsis transgenic lines expressing my protein of interest fused with GFP/YFP under the control of its native promoter. I want this for localization studies. Please...

23 January 2021 2,790 3 View

Queries regarding Antibody labelling ?

Hello Everyone. I was trying to label antibody with Cat# MO-L007 - Cystein reactive monolith series antibody labelling kit. I followed every steps that the protocol states. But in the final step...

26 September 2020 3,439 1 View

Is it possible solid state proton nmr with negative values?

I have done solid-state proton NMR. General solid-state proton NMR shows broad peaks. But, I find a negative value peak. In the case of solid-state, there should not use any reference (as...

13 July 2020 6,123 1 View

How do we calculate mechanical stress using VASP Calculations for Ab Initio Molecular Dynamics?

If we want to uniaxially strain a structure incrementally, and calculate the corresponding uniaxial stress at every strain point, how should we use the VASP output files for this purpose? I have...

04 April 2020 9,747 4 View

Can anyone please help me with ECV ( electrochemical capacitance voltage) profiling measurement?

I need to measure carrier concentration profile in phosphorus doped poly-crystalline silicon thin film. Is there any group who has the facility to perform ECV ( electrochemical capacitance...

29 February 2020 8,270 2 View

How do you model CO2 on VASP?

I am new to VASP, and I'm trying to model CO2. I was able to run the simulation and got all the output files, which I uploaded onto VESTA. However, VESTA shows that the bond length between the C...

23 January 2020 8,919 4 View

Can I use gromacs order to compute tetrahedral order parameter of water?

I want to calculate Orientational tetrahedral order parameter of water. The output of gmx_hydorder is confusing. can I use gmx_order instead?

17 January 2020 6,623 4 View

Cross section test to see whether second generation unit root test is required or not ?

I have a confusion. I had considered data in context of a particular nation in a paper and a reviewer suggested me to go for second generation unit root test instead of first generation unit root...

24 December 2019 9,239 1 View

What is the convenient way to to measure the degree of cooperativity in DNA-DNA or DNA-protein interaction?

Hill equation is used to determine the degree of cooperative behavior in ligand -enzyme interactions. If I would like to utilize this equation in DNA-DNA or protein-DNA interaction, is it...

18 December 2019 5,712 2 View

How to determine the box dimension for umbrella sampling?

I am using Gromacs 2016 to do umbrella sampling of a protein-RNA complex. I am following the Lemkul's tutorial of umbrella sampling, but I couldn't get how the box dimension was determined. For a...

24 April 2019 1,458 7 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT?

Im looking for any papers, reports,articles, links etc on information on How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT.

24 February 2021 2,750 4 View