Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

How to remove a sapphire disk from LR white resin embedding ?

Hello, We tried to do CLEM experiment on sapphire disk including cryo fixation (Leica ICE) and then cryo substitution using LR white resin. Once the resin is polymerized, we can't remove the...

13 January 2021 5,223 3 View

What do you suggest me as a low-cost electronic monitoring of honeybees using 3-5mg RFID tags?

We would like to control the honeybees' ID at the entrance of a flight tunnel. We would like to detect a honeybee in a cube of 10x10x10 cm3 in front of the tunnel door while flying, and thus...

17 December 2020 3,464 2 View

What are the competencies to put forward in Higher Education to prepare students to evolve in a world where IA is ubiquituous?

What are the competencies to put forward in Higher Education to prepare students to evolve in a world where IA is ubiquituous?

15 December 2020 6,350 4 View

Is it possible to invert a PLSR model, i-e to predict X based on Y?

There are many tools to build a PLSR model to predict a response variable Y based on a multivariate predictive variable X (reflectance spectra for example). My question is the following: once we...

06 December 2020 3,096 11 View

Have you ever included two last authors in the co-authorship of a publication?

Hello, As you must know, it is now sometimes possible to have several co-first authors in the co-authorship of a scientific publication, when two persons led the paper and had equal contribution...

21 September 2020 2,995 5 View

How does one find constants of the motion for a chaotic flow?

I'm reviewing a lot of papers where the authors take a 3-D autonomous chaotic system (think Lorenz) and add a fourth variable bidirectionally coupled to the other three and then report its unusual...

04 May 2020 3,785 33 View

Device abstraction for navigation in VR ?

Hello everyone The UMI3D Consortium's working group dedicated to embodiment is currently looking for a device agnostic way to manage navigation in Collaborative Virtual Environment (CVE). Are you...

08 April 2020 4,951 3 View

Redundancy analysis (RDA) order variables change result, why?

Hi everyone. I am in the process of making RDAs between faunistic and environmental tables. What I don't understand is that depending on the order in which I place my environmental variables...

29 March 2020 2,179 5 View

How to prepare fish (whole) in order to send it for analysis?

Hi, I have to collect fish, blend it, dry it and send it to a laboratory in order to have analysis of the fish composition. I need a whole fish, and I was wondering if there is a method to...

25 June 2019 1,884 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

No title

02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View