Could anyone bring me a solution?
I would like to know a goog transfection reagents to use with plasmids and mirna mimics together, looking the different lipofectamines I wonder if it is necessary to use for example the lipo 3000...
04 May 2017 7,212 2 View
I would like to clone my whole 3'UTR in a luciferase vector and I was wondering what it is better to use genomic sequences to obtain by PCR or to use RNA to obtain by RACE i I was reading that...
11 December 2016 348 0 View
I used genomatyx to analyze transcription factors in promoters sequences, but now I have many problems to login, so does anyone knows another facility to do this?
09 October 2016 6,052 5 View
Trying to express recombinant GST-proteins after overnight culture at 18ºC with 0.1 mM IPTG I observed also with my fusion protein a band corresponding to beta-lactamase after maldi analysis. How...
06 July 2016 9,515 5 View
I express a recombinant proteins in E. coli (wild type and mutant), the mutant one is much less expressed than the wild type and the culture is slower (time to attain DO 600 of 0.6 before to add...
09 October 2015 1,293 7 View
Sometimes it is not possible to see single bands in EMSA, and complexes remain as a big spot not resolved. Could anyone explain to me how to find a solution?
09 October 2013 3,655 5 View
I made cDNA constructions to produce proteins with GFP or dsRED (clontech), and one construction with dsRED after transfection in HeLa cells. I obtained RNA by qPCR, red cells by confocal...
31 December 2012 998 14 View
I bind my fused GST-protein to glutathion beads and I cut with Prescission at pH 7.5 to eluate overnight in batch method, sometimes my protein (always the same clone) remains attached to beads...
01 January 1970 6,443 5 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
