Bacterial Cellulose and Chitosan solution can mixed?

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How are Large Models Exploring and Outputting Knowledge Understanding in Specific Content Areas, and What Does Academic Research Say About It?

Hello everyone！ I am currently exploring the performance of large models in understanding knowledge in specific domains, and attempting to construct a knowledge framework similar to what...

05 August 2024 5,729 2 View

Is a reliability test necessary in my survey on translations?

Dear all, I gave 116 respondents 18 translated sentences and asked them to indicate their levels of acceptance of these translations on a five-point scale. Some translations result from strategies...

24 July 2024 8,245 5 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

Abnormal data in in-vitro drug release study?

I'm doing in-vitro drug release study using dialysis bag method and collecting samples from the external medium at several time points, subjecting to UV-Vis spectrophotometry. But my absorbance...

14 July 2024 6,829 2 View

Are my cells contaminated with mycoplasma?

I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...

11 July 2024 7,786 3 View

Black dots like contamination in my cell cultures. What are they?

There are black dots that seem like contamination in my corneal endothelial cell cultures. Some of the cells appear unhealthy and are enlarged. The black dots are more prevalent around these...

11 July 2024 4,829 0 View

What is the most simple language and character in the world?

Dear colleagues, As well known, we can deliver messages in different languages and characters, including Chinese, English, Latin, binary and decimal codes (if with a converter) and many other...

08 July 2024 5,422 13 View

After training an XGBoost model using K-fold cross-validation, Can I use SHAP to interpret this dataset?

In other words, I did not use the trained XGBoost model to make predictions on the test set and then use SHAP for interpretation. The reasons are as follows: Even with the best and most...

26 June 2024 7,332 1 View

Trouble shooting on mediation with survival regression and linear regression?

I did linear regression of X (independent variable) to M (Mediator) then I used survival regression to fit X to Y (dependent variable) With these questions: a. HOW to correctly do a mediation...

17 June 2024 1,478 6 View

Why is the blue and white spot screen negative for all white spots?

The protein was expressed using the insect cell system, and the recombinant plasmid was successfully constructed as verified by sequencing, and the recombinant plasmid was transformed into DH10...

13 June 2024 2,590 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Does anybody know why my chitosan/pva/gelatin hydrogel dissolve in PBS?

My final research is about making chitosan/pva hydrogel for wound healing using gelatin as crosslinker. Last month, i made the thin film hydrogel and it turn well. It was okay. Last week, i make...

28 July 2024 2,794 6 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

How can the rubber fibres produced by the electrospinning device be removed from the fibre collect(aluminium foil) ?

How can the rubber fibres produced by the electrospinning device be removed from the fibre collector (aluminium foil) without affecting the orientation of the rubber fibres for use in reinforcing...

26 July 2024 8,281 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View