Bacillus protoplast transformation with GFP plasmid pAD4325?

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Pascal Sartor

Hi Abdur, does it have to be a protoplast transformation? Which organism are you working with?

Karen A. Darbinyan

Hello, see below and attached info may be useful for you.

Article Transmating: Conjugative transfer of a new broad host range ...

https://www.mobitec.com/cms/products/bio/04_vector_sys/bacillus_megaterium_t7.html

Abdur Rashid Khan

Pascal Sartor yes, its Protoplast transformation. I am working with Bacillus isolates.