How to extract protein after trizol RNA and back extraction of genomic DNA?

I have limited amount of samples, so I am trying to get RNA, DNA and protein from the same samples. I used Trizol reagent for RNA isolation, then use back extraction buffer for genomic DNA...

13 January 2021 1,566 2 View

Where can I find solar irradiation data with high resolution?

I am trying to generate a 1 day solar irradiation profile. Any suggested high resolution(possibly in minutes) source for the same?

06 December 2020 5,976 18 View

How to implement only a Gate with activation sigmoid or tanh in keras?

I want to implement only a Gate similar to forget gate or output gate of LSTM cell. Mind it...!!! I am not implementing the entire LSTM cell rather I have certain input and previous output and I...

22 September 2020 8,236 3 View

Could someone help me interpret the following PMF curve, especially the physical reason behind the multiple potential wells?

I am simulating the pulling of two nanoparticles in a polymer. I get the attached PMF curve, showing a secondary (and much deeper) potential well. I was wondering if there is a physical...

19 September 2020 7,504 5 View

How to determine relationship among the independent variables to get lowest MSE (Mean Squared Error) in prediction?

I have a dependent variable A, which can be perceived from B, C and D. So to say A = function(B,C,D). B, C, D results in A that is known to us. However, I don't know whether B,C and D follow a...

28 June 2020 6,332 6 View

IS it Necessary for Primers in Taq-Man PCR to be HPLC Purified?

I want to set up a taq man reaction. I want to know that in taq man reaction I have to order HPLC purified Primers or ordinary primers are adequate for me. If you have experience in taq man,...

23 June 2020 5,511 3 View

No title

We're looking at storing serum and plasma samples from a clinical trial. I've looked at various papers, but the only place where I found some hazy answer was in the supplementary. But I'm hoping,...

04 March 2020 8,858 3 View

How to calculate the required motor HP to run a machine?

Hello every one, Can some body help me in calculating the required HP for motor to run the machine which includes transmission system with 2 Nos of shaft and cutting blades.

28 December 2019 9,788 3 View

Is there any benchmark dataset for distributed camera networks?

I am particularly looking for dataset containing cameras capturing videos in a distributed setup. The videos, captured by multiple cameras have to be correlated. Is there any benchmark dataset on...

22 November 2019 9,567 3 View

Could anyone recommend a protocol to estimate the concentration of residual antibiotics in a tissue allograft?

I have been trying to detect and quantify residual antibiotics left over from processing of my tissue allografts. Could anyone help me with the same?

01 August 2019 8,465 4 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

Historical wind data/map?

Hello All, I am looking for the historical wind data/map (mainly the year 2020-2021). I would like to look at the wind value and direction for the specifics locations within the US for the...

18 February 2021 9,543 4 View

I need a Wind Power Dataset having wind turbine's output power values and corresponding weather data information: wind speed, pressure, temperature?

The dataset is needed for forecasting wind power output using the weather information. I will be much obliged if anyone can share a relevant dataset or link.

04 January 2021 6,344 2 View

Can anyone share machine (generator) data for IEEE 30 bus system?

I require the machine (generator) data for H, Rs, Xd, Xtd, Xq, Xtq, Td0, Tq0 and D; as well as the exciter data (kA, TA, kE, TE, kF and TF). If you can share the machine data file or link, it will...

22 December 2020 2,384 3 View

How weather based insurance is more effective than yield based?

In Nepal, crop insurance and refunding policy is is area-yield /revenue-based and cost of production based. Farmers are partially following crop insurance in comparison to livestock and other life...

07 December 2020 2,127 1 View

How could you explain that weather is a factor with such contribution in keeping the same level of GHG emissions?

" The IEA report looks at changes in greenhouse gas emissions between 2018 and 2019. It finds that energy-using activities increased in 2019, but the emissions did not increase because of a...

03 December 2020 7,073 3 View

How to determine/select the indices (underlying weather index) of weather-based insurance?

It should not be confused with strike and limit thresholds. In this case, I am emphasizing the basis of the index, such as: -Cumulative, accumulated, deficit and excess rainfall values. -Average,...

29 November 2020 3,084 3 View

Which time-serie model is good when using concatenate data?

I would like to make a model using sequence datasets. As I've searched that , many LSTM, RNN tutorials use long-sequential data such as weather in 1950-2020. However, my datasets are short...

24 November 2020 808 3 View

Single-phase synchronous machine models?

Hi there, In low frequency railways it is common with single-phase synchronous machines. Anyone that knows how to model them dynamically in a trustworhy manner? Moreover, there are many...

18 November 2020 1,357 5 View

How to calculate UTCI using ERA5 dataset?

17 November 2020 2,127 3 View