How we can calculate Dn?
Average duration required for germination :
ADG=Dn/n
Where, ADG= Average duration required for germination
n= number of seeds
Dn= number of seeds germinated in days
MTG = Average germination time
n = The number of seeds sprouted on Saturday
D = Number of days after germination
ref :
Salehzade H, Izadkhah Shishvan M, Chiyasi M (2009) Effect of seed priming on germination and seedling growth of wheat (Triticum aestivum L.). Journal of Biological Sciences 4(5): 629-631.
Dn = n x ADG
MGT Mean germination time
MGT= sum di ×ni/sum ni
ni Number of seeds germinated per day i
di day i after the test begins
i first day, second ..
56 hours for radicle protrusion
Av. duration of germination = Sum (n.t)/ Sum n where t is time and n is no. of germinates
Do you mean MGT (Mean germination Time)?
Its an In Vitro Cell Proliferation using Leukemia patient cells lines. Cell Proliferation Dye Used : Tag-It Violet Cell Proliferation Dye. Thank you a lot.
09 June 2024 1,194 1 View
I check the purity of commercially available protein (casein) by HPLC and SDS. HPLC giving intense beta casein peak and low alpha casein peak where as on SDS gel show two resolve bands with almost...
09 January 2024 6,300 0 View
I need the specific concentration of different components to study the effects of carbon and nitrogen sources on CaCO3 precipitation.
19 September 2023 6,590 0 View
Effect of Microplastics on the Germination and Growth of Mangrove Plants
17 September 2023 9,376 0 View
I synthesized protein nanomaterials using different divalent metal ions, and intrinsic Fluorescence intensity was measured, my results show nanomaterials with Copper and ferrous ions significant...
01 September 2023 9,272 1 View
Actually I am having issue with double purified PCR bands for gel electrophoresis..
29 August 2023 7,717 5 View
I used a new column, C18, but there are peaks in the blank. I use ammonium acetate (o.1M pH 5) and acetonitrile buffers A and B respectively for dye separation. and did several washings but the...
21 June 2023 7,206 1 View
I checked salts' effect on methylene blue degradation utilizing protein-copper-based nanomaterial. since it was a literature report that chlorine ion could reduce degradation rate but in my case,...
15 June 2023 1,280 1 View
I degraded Methylene blue dye with copper base nanoparticles. but they degraded the MB in 24 hours. so should I do kinetics and relative graph by giving a 1-hour difference? and I want to...
29 December 2022 7,128 11 View
I followed the degradation of dye till 24 hour .. so how I should calculate rate constant because i take UV with a gape of 1 hour. what its unit will be?
29 November 2022 4,097 2 View
I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...
02 August 2024 1,178 3 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,301 7 View
Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...
24 July 2024 8,850 4 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.
11 July 2024 9,045 4 View
I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...
10 July 2024 7,145 2 View
I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...
07 July 2024 407 0 View
I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.
01 July 2024 8,361 3 View
Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...
30 June 2024 9,300 0 View