I synthesized protein nanomaterials using different divalent metal ions, and intrinsic Fluorescence intensity was measured, my results show nanomaterials with Copper and ferrous ions significant quenching while other metals showed significant increase intensity like with zinc cobalt and megnease as compared to control alone. like I used 0.05mg/ml protein in my synthesis. why did this happen?
Depending on what metal ion in what charge state you have, a completely different ligand field occurs. Are you familiar with ligand field theory, the nephelauxetic effect and Tanabe-Sugano diagrams? If not, please check out an introductory textbook (or youtube tutorial) on these terms.
Depending on the set of electronic states you have available, different transitions are symmetry-allowed or forbidden within your complex or at least the oscillator strengths vary substantially, thus pushing the energy from your excitation into different channels.
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