Does CYTO-ID kit works with mouse erythroid cells cytospined directly on the slides?
Any suggestion, how to stain live cells using ENZO CYTO-ID kit for confocal imaging? ? ?
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
Hello, Currently, I am writing my master thesis proposal. I have been to provide a method paper for (question creation and interview evaluation). It's my first time planning to conduct...
27 April 2024 4,710 1 View
I am hoping that someone can help me find an antibody for GABA, GAD 65 or GAD 67 that works well in marmoset cerebral cortex Thanks
21 February 2024 7,493 2 View
Hello, I am looking for a dataset of requirements gathering scripts and user stories extracted from those requirements. Anything similar would be helpful. Thank you.
01 February 2024 4,604 0 View
Hello , I hope this message finds you well. I'm a final-year civil engineering student working on a dissertation about the sustainability of ancient Egyptian construction techniques and their...
14 November 2023 987 1 View
I used some polyclonal western blot antibody, and that datasheet size is 45-50kDa I also get the band in expected size range, but there's multiple other band(at least 3 bands in other size), and...
25 July 2023 5,349 5 View
Hi! I am trying to run a model 4 mediation with the xmint option (what was once model 74) but PROCESS produces the following error: "A reference value of X is required for this model." I've...
24 May 2023 6,965 2 View
Hello, I would some help with seeding/plating cells. I counted cells using the hemocytometer. I got 1,025,000 cells per mL. I need to seed 250 cells per well for 36 wells and each well has...
26 March 2023 2,481 3 View
what is the recomended concentration and duration to permeabilize mitochondrial membrane for ICC?
26 March 2023 1,958 9 View
I am studying the subcellular localization of a protein. To see if a GFP fusion protein can be compared to the native protein, I fused GFP to the N-terminal of the protein. In my western blots and...
01 March 2023 749 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View