How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Anchoring zone/port statistics numbers?

Hello everybody, For one of my Ph.D. research papers, I'm looking for statistical periodical (annual and monthly 2012-2020) datasets of port statistics: number of ships, average waiting time, type...

15 December 2020 9,128 3 View

Most of the plastic is shipped to Asia?

Dear Sir I came across your paper. First of all, congratulations on your amazing research article. Loved it! Also, I am very interested to know, why is the waste that is collected in the...

14 December 2020 6,342 4 View

Recommended insect storage/shipping method for NGS ?

Dear all. We need to sample insects, store them upon collection and ship them internationally. Sampled will later be processed for DNA extraction and NGS sequencing. Keeping all this in mind, can...

25 October 2020 7,390 2 View

How can I determine the lift and drag force on a planing boat from the heave and surge forces experimentally determined by a load cell in towing tank?

The heave and surge forces on a planing boat model was measured in a towing tank. Can resolution of these forces with respect to the direction of motion of the model yield the resultant lift and...

05 October 2020 7,576 1 View

DNA extraction of swabs stored in ethanol?

Hi all, I am using buccal swabs of seals as a sample source for microsats and mitochondrial sequencing. There is a few samples that were shipped stored in ethanol. , i.e. swab of the buccal area...

08 September 2020 1,013 1 View

No title

Fluid mechanics

20 July 2020 7,656 27 View

Best open source software for wave interaction with floating solar array in a lake?

I know the analysis of wave interaction with the floating bodies can be done in the ansys aqwa but it is a paid product and I am looking for a free option. However, I have the license of MIKE MA,...

05 May 2020 9,600 2 View

If you have ever taken a holiday on a cruise ship, what are your TOP THREE reasons for doing so?

I am currently in the midst of a wider study on the motivations for cruise tourism. COVID-19 has been devastating for the image of cruises and I am keen to understand whether this will have any...

21 April 2020 7,652 5 View

How do you identify the complete added mass matrix of a ship using experimental data?

I am looking for a purely experimental method to determine the mass matrix of a ship in three degrees of freedom using manoeuvres in open water. I just found: Skjetne, Smogeli, Fossen: The...

23 March 2020 814 1 View

Acoustic Emission signals characteristic signatures?

I am doing a study on AE signals characteristic signatures, where each defect would give unique wave form of AE signals. These signatures became a standard tool for AE data interpretation and...

10 March 2020 4,893 4 View