I have two anticancer drugs that are used to have a concentration of drug A (1 mg/kg) and drug (5 mg/kg) from literature. I need to combine these two drugs and load them in a nanoparticle for i.v. injection in 4T1-bearing BALB/c mice.
My questions are:
1) At which concentration I need to adjust these drugs in the combination?
2) how to decide the injection periods?
3) how to decide the injection volume?
Thanks.
Dear Majdi Al-Amili,
What is the aim of this animal study? Investigation of toxicity, pharmacokinetics or pharmacodynamics of the combination?
Kind regards
Dear Majdi,
Even if you are going to start at 1:5 ratios of the two drugs when you prepare the NP, you most likely won't end up with a final ratio of 1:5 encapsulated. Thus you will need to optimize the protocol to produce the final NP with a ratio of 1:5 encapsulated. Then simply you inject an equilavent dose of drugs.
For example, if for free drugs you will use 1 and 5 mg/kg respectively, you will have to inject the same dose with the nano ( a concentration of nano that has 1mg/kg of drug 1 and 5mg/kg of drug 2). For this, it is critical to produce the nano with 1:5 final ratio.
I hope this helps.
Regards
Francesco
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