How to look hany receptors have been endocytosised by western blot?

I am trying to do DDR1 receptor endocytosis test. I use collageI coated fask to activate DDR1 and want to know if DDR1 will be phophorylated and be endocytosed in to the cell. how can I exclude...

07 August 2018 1,003 2 View

Lipid tube fixation?

Hello, everyone, I amworking on a lipid tube, when transfect the lipid probe to show the lipid tube, we can observe it very clearly under microscope for live cell imaging. But when fixed the dish...

03 April 2018 9,489 1 View

DDR1 activation by CollageI

Dear experts, I am trying to detect phospho-DDR1 after collageI stimulation, I tried to use 50ug/ml collageI in PBS coating the dish at 37 degree for 1-2hour before seeding cells. And then try...

01 January 1970 9,598 0 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

No title

02 March 2021 324 2 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View