26 October 2018 6 3K Report

the new findings on stem cells and their applications

More Ackah Isaac's questions See All
Background on the project topic gender and sports participation in cape coast metropolitan?

an introduction

02 June 2024 520 0 View

Thesis on the effect of stress on employees performance and productivity in Ghana?

I will be grateful if i can get the latest work on the above topic.

17 May 2024 2,509 1 View

How much DNase I is too much?

I am using the Thermo Scientific DNase I (https://www.thermofisher.com/order/catalog/product/EN0521) to remove genomic DNA from RNA extracts eluted in nuclease free water. The protocol calls for...

30 March 2024 7,100 0 View

Arcswat: There's is no overlap with the watershed grid?

My watershed keeps showing there's no overlap with the watershed grid and I have clearly projected it in the same projection as the watershed and source dem. WGS 84, UTM 32. But when try to use...

18 February 2024 1,304 1 View

Autocorrelation descriptors in 3d QSAR?

i would like to ask, wheather autocorrelation desciptors are sub type of 2d or 3d desciptors , they are offered by many platforms to calculate without demon about the differnce between them and...

09 December 2023 8,714 3 View

MacOS or online apps to make sketches of molecular interactions on 2-D?

There's a great app called ChemSketch but only compatible with Windows. I'm looking for similar options for a Mac user. Thnks!

29 November 2023 3,237 2 View

How to calculate UFC/mL from a non-diluted sample?

I did an experiment where I evaluated the growth of colonies of a bacteria in different samples. For one of them it was not necessary to do serial dilutions. In this case my dilution factor cannot...

19 November 2023 3,608 8 View

Error with autodock 4 gld file?

hallo everybody , iam using autodock vina for small molecule docking , however after finalizing everything with the grid the running of autogrid cannot be predeeded and no gld file is created with...

04 November 2023 5,952 2 View

In spectrophotometry readings, does anyone know the difference between Absorbance and Standard Concentration readings?

I'm working with a Thermo Scientific GENESYS 10S Series UV-Visible Spectrophotometer. I took readings of a cell culture in two different modes: Absorbance (595 nm) and Conc/Std (Factor = 0.745,...

16 October 2023 2,898 1 View

Does anyone know the reason why some microbial growth curve protocols read optical density readings at 595 nm and others at 600 nm?

What is the difference? Does it affect viability? If there is a difference in measurement, is it significant?

15 October 2023 729 2 View

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What are the emerging trends and opportunities for STEM education and careers in the online space?

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

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How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

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