How may i find a researcher who is working on Teachers Engagement?

Hi everyone, my current Ph.D Project is on "Teachers Engagement in STEM teaching" Please I like to link up with (find) another or any researcher working on Teachers Engagement. I will be grateful...

14 January 2021 8,170 5 View

What Explained Variance is good to proceed with Predictive Soil Mapping?

I have 1851 soil samples data on pH covering a study area of 7482sq.km in northern Ghana and I am using 52 environmental long-term average variables (Relief, Climate, MODIS Reflectances and...

27 December 2020 2,728 6 View

What is the difference between PET28a(+) 6595bp and PET28a(+) 5369bp?

I am doing restriction cloning, and I want to choose a restriction enzyme and clone my target gene into a PET28a protein expression vector. I'm confused about which of the two vectors to use since...

12 November 2020 5,378 3 View

How do I determine DNA of 1 ug?

I am currently working on DNA methylation and I needed to determine 1ug of the extracted DNA sample to run my MSAP analysis. I'm stuck on how to get the this done and I need help

04 October 2020 6,600 4 View

How did you feel when you received a notification of the acceptance of your first paper?

As a novice in the world of research with the aim to publish in a peer-reviewed high-quality journal, you do all you can to get quality work. Sometimes the feedback from reviewers can discourage...

09 September 2020 147 31 View

Is it possible to get DOI for previous publications without DOIs?

Have previous publications without DOI with certain journals though they now provide DOIs but don't seems to be ready to link up for the provision of DOIs for previous publications. Any means to...

31 August 2020 9,773 5 View

Reference style "Games for Health Journal"?

Does anybody knows if there is a reference style in Mendeley for "Games for Health Journal" ISSN 21617856, 2161783X?

09 July 2020 8,151 1 View

How do i get a cancer cell and standard procedure for storage?

I may need a standard procedure of storage

05 April 2020 163 3 View

OK to store pelleted transformed E coli at 4C for 2 days?

Immediately after transforming competent cells with a plasmid ordered from Addgene, I began growing a mini prep culture (5ml) overnight. Next morning, I pelleted the cells and put them in the...

01 February 2020 2,085 2 View

What is the primary structure of a typical keynote address usually delivered at a conference?

Keynote address

07 September 2019 567 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View